Hotshot extractions
First Time Hotshot Extractions
Samples:
| Tube # | Sample # |
|---|---|
| 1 | 10-0173 |
| 2 | 10-0174 |
| 3 | 10–113 |
| 4 | Thenus#001 |
| 5 | 10-0183 |
| 6 | 10-0143 |
| 7 | 10-0140 |
Materials:
- water (MilliQ)
- 10% Bleach
- 1 M Tris-HCL (pH 8.0)
- 50 mM NaOH
- strip tube
- forceps
- razor blade
Procedure:
- Collect tissue from sample (pencil tip sized amount)
- Fill tube with 100uL NaOH
- Place tissue in tube with NaOH
- Put tubes in PCR machine and use hotshot setting (25 ºC for 25 min)
- add Tris (10% = 10 uL) into tubes
- place strip tube in plate and place in fridge
Notes: clean workspace with bleach after each sample and replace pipette tip
Written on October 12, 2020