Gel and Dilutions
Run 1.5% gel with dilutions made 10-26-2020
I’m assuming this didn’t work either?
Prep new PCR samples to test primers - controls
| Tube # | Sample # |
|---|---|
| 1 | PM-16 |
| 2 | PM-17 |
| 3 | PM-18 |
| 4 | PM-19 |
| 5 | 10-009 |
| 6 | 10-0011 |
| 7 | Fiji 015 |
| -C | water |
Thermalcycler protocol
| Temp | Time | | ———- | —- | |Denaturation| | | 95 ºC | 4:00 | |Annealing | x35 | | 95 ºC | 0:30 | | 48 ºC | 0:45 | | 72 ºC | 0:45 | |Extension | | | 72 ºC |10:00 | | 15 ºC | inf |
2 strip tubes: one with HCO/LCO primers, and the other with BAR primers
Written on November 2, 2020