Finish BIOL 3071 - Gel and Sequencing
Gel for remaining BIOL 3071 samples
1.5% gel = 1.25 g agarose + 150 mL TAE + 7.5 ul smart glow
8 ul in each well load at 2:30 run at 2:45 done at 3:15
First Row | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | |——|——|——|——|——|——|——|——|——| |SBOH -|SBOH +| MP ? | EMP 3| EMP 2| SDN | SEJ -| SEJ +| SEJ |
Second Row | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | |——|——|——|——|——|——|——|——|——| | MMO | GR - | ?? + | GR 2 | CH 1 | NA | NA - | IJ - | YF 2 |
Group 1 (from 11/9/20)
| + | - | fish samples | | —– | —– | ———— | |KKBW + |KKBW - | BW | |JGJZ + |JGJZ - | KK | | MK + | MK - | 2 | | AMK + | SMS - | SMS1 | | AMY + | KT - | AMK1 | | MES + | AES - | AMY | | MB + | AMK - | JRP 2 | | | | KT | | | | AKB, MK | | | | AS(Isabelle?)| | | | JTZ 1 | | | | JMG | | | | JM, MK | | | | JMB | | | | DH | | | | AES (Anna S) |
Group 2 (from today)
| + | - | fish samples |
|---|---|---|
| SBDH + | SBDH - | MP ? |
| SEJ + | SEJ - | EMP 3 |
| + | GR - | EMP 2 |
| NA | NA - | SDN |
| SEJ | ||
| MMO | ||
| YF2 | ||
| GR2 | ||
| CH1 | ||
| IJ1 | ||
| JTZ 1 | ||
| JMG | ||
| JM, MK | ||
| JMB | ||
| DH | ||
| AES (Anna S) |
Prepare samples for sequencing
5 ul PCR product, 2 ul EXOFAP into new strip tube
| Tube # | Sample # |
|---|---|
| 1 | KK |
| 2 | BW |
| 3 | 2 |
| 4 | SMS1 |
| 5 | AMK 1 |
| 6 | AMY |
| 7 | AS |
| 8 | SMB |
| 9 | JMG |
| 10 | JTZ 1 |
| 11 | AES |
| 12 | DH |
| 13 | JMB,MK |
| 14 | JRP 2 |
| 15 | KKBW + |
| 16 | KKBW - |
| 17 | MK, AK |
| 18 | KT |
| 19 | SDN |
| 20 | EMP 2 |
| 21 | SEJ |
| 22 | MMO |
| 23 | YF 2 |
| 24 | CH 1 |
| 25 | IJ 1 |
| 26 | GR 2 |
| 27 | SEJ + |
| 28 | SBDH + |
Thermocycler protocol: EXO
| Temp | Time |
|---|---|
| 37 ºC | 1:00 |
| 85 ºC | 0:15 |
| 15 ºC | inf |