small gel for lobster samples: 1.5% = 1.5g agarose, 100 mL TAE

• BAR primer samples (see 11-2-2020) on top
• HCO/LCO samples on bottom
• Run 85V at 12:00 pm, view at 12:38 pm

Results: BAR primers worked, HCO/LCO did not

medium gel for fish (BIOL 3071): 1.5% = 2.25g agarose, 150 mL TAE

8ul in each well

First Row | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | |——|——|——|——|——|——|——|——|——|——|——|——|——|——|——|——| | BW |KKBW 1|KKBW +| KK | JG - | JG + | JTZ 1| JMG | 50bp | MK + | MK - | JMMK | AMKK | 2 | SMS 1| SMS -|

Second Row | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | |——|——|——|——|——|——|——|——|——|——|——|——|——|——|——|——| | MB + |AMK 1 |AMK - |JRP 2 |AMK + |AES - |AES + | AES | 50bp | AS | KT | AMY |RMY + | KT - | DH | SMS |

Qubit

Make sure all liquids are room temp

• 1:199 ratio reagent to buffer = working solution
• to make standards: 190 uL working soln., 10 uL standard in Qubit assay tubes
• to make samples: 5 uL sample, 195 ul working soln. in Qubit assay tubes

For samples from 11-2-2020, (controls):

• working soln. 9 samples = 9 uL reagent, 1791 uL buffer
• vortex each sample very briefly and spin down

Results: